Selenium accumulation in mycelia of Flammulina velutipes during fermentation determined by RP-HPLC.

Optimization of soluble organic selenium accumulation during fermentation of Flammulina velutipes mycelia.

Selenium is an essential nutrient with diverse physiological functions, and soluble organic selenium (SOS) sources have a higher bioavailability than inorganic selenium sources. Based on the response surface methodology and central composite design, this study presents the optimal medium components for SOS accumulation in batch cultures of Flammulina velutipes, i.e. 30 g/L glucose, 11.2 mg/L so...

Development of a gene transfer system for the mycelia of Flammulina velutipes Fv-1 strain.

To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter. The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes. Transformation efficiency was much improved when transformation was performed by the restriction enz...

Selenium uptake, tolerance and reduction in Flammulina velutipes supplied with selenite

Recently, selenium (Se) enriched mushrooms have been exploited as dietary Se supplements, but our knowledge of the metabolic process during the Se enrichment process is far from complete. In this study, the uptake, tolerance and reduction of selenite in a widely cultivated mushroom, Flammulina velutipes, was investigated. The results showed that pH variation (from 5.5-7.5), metabolic inhibitor ...

Selenium uptake, toxicity and reduction in Flammulina velutipes supplied with selenite

RELATIONSHIP BETWEEN THE SELENIUM, SELENOMETHIONINE, AND SELENOCYSTEINE CONTENT OF SUBMERGED CULTIVATED MYCELIUM OF Lentinula edodes (Berk.)

To obtain extracts rich in the organic forms of selenium with putative cancer-preventive properties more effective than those of selenizedyeast, Lentinula edodes mycelia were cultivated in media enriched with selenium at concentrations from 0 to 20 μg mL, by addition of sodium selenite either before inoculation or after three days of mycelial growth. The total selenium content of submerged cult...